bd difcotm brain heart infusion agar  (Difco)

Journal: Frontiers in Microbiology

Article Title: Corynebacterium Species Inhibit Streptococcus pneumoniae Colonization and Infection of the Mouse Airway

doi: 10.3389/fmicb.2021.804935

Figure Lengend Snippet: Inhibition of S. pneumoniae growth in vitro by lipophilic and non-lipophilic Corynebacterium species. Supernatant inhibition assay schematic using tryptic soy broth (TSB), neomycin (Neo), and catalase plates (A) . Growth of type 2 S. pneumoniae (Spn), (B) , type 4 Spn (C) , and type 3 Spn (D) on untreated plates (–) or on plates pre-treated with supernatants from C. accolens ( C. acc ) or C. amycolatum ( C. amy ) or with 180 mg/mL oleic acid. Growth of type 2 Spn on untreated plates (–) or on plates pre-treated with supernatants from C. acc or C. amy grown in BHI with or without 1% Tween 80 and 180 mg/mL triolein (E) . Limit of detection (LOD) is indicated. Data are pooled from three independent experiments in duplicate. *** p < 0.001, ANOVA.

Article Snippet: Corynebacterium strains were grown from glycerol stocks on BHI agar plates (BD DifcoTM BactoTM Brain Heart Infusion, Thermo Fisher Scientific) aerobically at 37°C overnight.

Techniques: Inhibition, In Vitro

Journal: Frontiers in Microbiology

Article Title: Corynebacterium Species Inhibit Streptococcus pneumoniae Colonization and Infection of the Mouse Airway

doi: 10.3389/fmicb.2021.804935

Figure Lengend Snippet: Corynebacterium lipases contribute to, but are not required for, protection against S. pneumoniae in vivo. Growth of type 2 S. pneumoniae (Spn) on untreated plates (–) or on plates pre-treated with supernatants from WT vs. ΔlipS1 C. acc or WT vs. Δlip C. amy grown in BHI with 1% Tween 80 and 180 mg/mL triolein (A) . Burden of Spn (B) or Corynebacterium ( Coryneb ) (C) in nasal lavage fluid and lungs of mice pre-exposed to PBS (–), WT C. acc or ΔlipS1 C. acc at 24 h post-Spn infection detected by live growth. Limit of detection (LOD) is indicated. Data are pooled from three independent experiments with 3–5 mice per group. * p < 0.05, ** p < 0.01, *** p < 0.001, ANOVA (A) or Kruskal-Wallis test (B,C) .

Article Snippet: Corynebacterium strains were grown from glycerol stocks on BHI agar plates (BD DifcoTM BactoTM Brain Heart Infusion, Thermo Fisher Scientific) aerobically at 37°C overnight.

Techniques: In Vivo, Infection